Journal: Journal of Nanobiotechnology
Article Title: Finely tuned ionizable lipid nanoparticles for CRISPR/Cas9 ribonucleoprotein delivery and gene editing
doi: 10.1186/s12951-024-02427-2
Figure Lengend Snippet: Effect of pH on the uptake and gene editing efficiency of CrLNPs. Confocal microscopy of A , B HEK293T cells and C , D various cell lines (CT26, 4T1, DCON, RENCA, Raw264.7) treated with CrLNPs to examine cellular uptake (scale bar: 10 μm for A , 40 μm for C ). B , D Quantification of fluorescence by image analyses. Data are shown as mean ± SD, * P < 0.05, *** P < 0.001, **** P < 0.0001 by student’s t -test. E Schematic of CrLNPs treatment to HEK293T cells expressing surrogate reporter and analyses by flow cytometry. Gene editing efficiencies of HEK293T determined by F flow cytometry and G confocal microscopy. Scale bar, 40 μm
Article Snippet: CT26 murine colon cancer cells, 4T1 murine breast cancer cells, RENCA murine kidney cancer cells, Raw264.7 murine macrophage cells, and HEK293T human embryonic kidney cells were obtained from the American Type Culture Collection (ATCC).
Techniques: Confocal Microscopy, Fluorescence, Expressing, Flow Cytometry