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Procell Inc kidney cancer renca
Kidney Cancer Renca, supplied by Procell Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Effect of pH on the uptake and gene editing efficiency of CrLNPs. Confocal microscopy of A , B HEK293T cells and C , D various cell <t>lines</t> <t>(CT26,</t> 4T1, DCON, <t>RENCA,</t> Raw264.7) treated with CrLNPs to examine cellular uptake (scale bar: 10 μm for A , 40 μm for C ). B , D Quantification of fluorescence by image analyses. Data are shown as mean ± SD, * P < 0.05, *** P < 0.001, **** P < 0.0001 by student’s t -test. E Schematic of CrLNPs treatment to HEK293T cells expressing surrogate reporter and analyses by flow cytometry. Gene editing efficiencies of HEK293T determined by F flow cytometry and G confocal microscopy. Scale bar, 40 μm
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Effect of pH on the uptake and gene editing efficiency of CrLNPs. Confocal microscopy of A , B HEK293T cells and C , D various cell <t>lines</t> <t>(CT26,</t> 4T1, DCON, <t>RENCA,</t> Raw264.7) treated with CrLNPs to examine cellular uptake (scale bar: 10 μm for A , 40 μm for C ). B , D Quantification of fluorescence by image analyses. Data are shown as mean ± SD, * P < 0.05, *** P < 0.001, **** P < 0.0001 by student’s t -test. E Schematic of CrLNPs treatment to HEK293T cells expressing surrogate reporter and analyses by flow cytometry. Gene editing efficiencies of HEK293T determined by F flow cytometry and G confocal microscopy. Scale bar, 40 μm
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Effect of pH on the uptake and gene editing efficiency of CrLNPs. Confocal microscopy of A , B HEK293T cells and C , D various cell <t>lines</t> <t>(CT26,</t> 4T1, DCON, <t>RENCA,</t> Raw264.7) treated with CrLNPs to examine cellular uptake (scale bar: 10 μm for A , 40 μm for C ). B , D Quantification of fluorescence by image analyses. Data are shown as mean ± SD, * P < 0.05, *** P < 0.001, **** P < 0.0001 by student’s t -test. E Schematic of CrLNPs treatment to HEK293T cells expressing surrogate reporter and analyses by flow cytometry. Gene editing efficiencies of HEK293T determined by F flow cytometry and G confocal microscopy. Scale bar, 40 μm
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Effect of pH on the uptake and gene editing efficiency of CrLNPs. Confocal microscopy of A , B HEK293T cells and C , D various cell <t>lines</t> <t>(CT26,</t> 4T1, DCON, <t>RENCA,</t> Raw264.7) treated with CrLNPs to examine cellular uptake (scale bar: 10 μm for A , 40 μm for C ). B , D Quantification of fluorescence by image analyses. Data are shown as mean ± SD, * P < 0.05, *** P < 0.001, **** P < 0.0001 by student’s t -test. E Schematic of CrLNPs treatment to HEK293T cells expressing surrogate reporter and analyses by flow cytometry. Gene editing efficiencies of HEK293T determined by F flow cytometry and G confocal microscopy. Scale bar, 40 μm
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Effect of pH on the uptake and gene editing efficiency of CrLNPs. Confocal microscopy of A , B HEK293T cells and C , D various cell <t>lines</t> <t>(CT26,</t> 4T1, DCON, <t>RENCA,</t> Raw264.7) treated with CrLNPs to examine cellular uptake (scale bar: 10 μm for A , 40 μm for C ). B , D Quantification of fluorescence by image analyses. Data are shown as mean ± SD, * P < 0.05, *** P < 0.001, **** P < 0.0001 by student’s t -test. E Schematic of CrLNPs treatment to HEK293T cells expressing surrogate reporter and analyses by flow cytometry. Gene editing efficiencies of HEK293T determined by F flow cytometry and G confocal microscopy. Scale bar, 40 μm
Renca Mouse Kidney Cancer Cell Line, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Effect of pH on the uptake and gene editing efficiency of CrLNPs. Confocal microscopy of A , B HEK293T cells and C , D various cell lines (CT26, 4T1, DCON, RENCA, Raw264.7) treated with CrLNPs to examine cellular uptake (scale bar: 10 μm for A , 40 μm for C ). B , D Quantification of fluorescence by image analyses. Data are shown as mean ± SD, * P < 0.05, *** P < 0.001, **** P < 0.0001 by student’s t -test. E Schematic of CrLNPs treatment to HEK293T cells expressing surrogate reporter and analyses by flow cytometry. Gene editing efficiencies of HEK293T determined by F flow cytometry and G confocal microscopy. Scale bar, 40 μm

Journal: Journal of Nanobiotechnology

Article Title: Finely tuned ionizable lipid nanoparticles for CRISPR/Cas9 ribonucleoprotein delivery and gene editing

doi: 10.1186/s12951-024-02427-2

Figure Lengend Snippet: Effect of pH on the uptake and gene editing efficiency of CrLNPs. Confocal microscopy of A , B HEK293T cells and C , D various cell lines (CT26, 4T1, DCON, RENCA, Raw264.7) treated with CrLNPs to examine cellular uptake (scale bar: 10 μm for A , 40 μm for C ). B , D Quantification of fluorescence by image analyses. Data are shown as mean ± SD, * P < 0.05, *** P < 0.001, **** P < 0.0001 by student’s t -test. E Schematic of CrLNPs treatment to HEK293T cells expressing surrogate reporter and analyses by flow cytometry. Gene editing efficiencies of HEK293T determined by F flow cytometry and G confocal microscopy. Scale bar, 40 μm

Article Snippet: CT26 murine colon cancer cells, 4T1 murine breast cancer cells, RENCA murine kidney cancer cells, Raw264.7 murine macrophage cells, and HEK293T human embryonic kidney cells were obtained from the American Type Culture Collection (ATCC).

Techniques: Confocal Microscopy, Fluorescence, Expressing, Flow Cytometry